Invasive alien species (IAS) are a major threat to biodiversity, often causing irreversible changes to ecosystem structure, composition, and functioning. They can also adversely affect local communities that depend on ecosystem services for their livelihoods. In addition, invasive species impose substantial economic costs, with estimates indicating that their annual impact on just six countries, namely the United States, United Kingdom, Australia, South Africa, India, and Brazil, amounts to approximately US$336 billion (Pimentel et al., 2001).Botswana is particularly vulnerable to invasions by alien plant species due to its proximity to South Africa, where more than 350 invasive plant species have been recorded. This project seeks to assess the distribution, abundance, and ecological impacts of invasive alien plant species in the Kgalagadi and Ghanzi districts of Botswana. The findings will provide critical information for the development of effective monitoring, prevention, control, and management strategies. Furthermore, the results will support the formulation of comprehensive national policies and regulatory frameworks to govern the introduction, cultivation, trade, and management of invasive alien plant species in Botswana.