Despite treatment with antiretroviral drugs, HIV-infected women have higher risk of cervical cancer, and are often diagnosed at advanced stages, with reduced survival. However, the progression of cervical cancer during HIV infection is not completely understood. During HIV infection, HIV-infected T cells release biological messengers known as exosomes, which carry cargos to affect target cells. We propose that during HIV infection, exosomes are enriched with HIV-derived molecules and inhibitors of anticancer immune function to promote cervical cancer progression. We will analyze exosomes isolated from plasma samples of 48 cervical cancer patients, 12 women with precancer and 12 women without cervical cancer, each group composed of women with or without HIV infection. We will quantify the exosomes, their levels of HIV TAR RNA using RT-qPCR and their levels of markers of immune dysfunction using Western blotting. We anticipate that circulating exosomes, exosomal HIV TAR RNA, and immune dysfunction markers will increase with precancer, invasive cancer, cancer stage, and further increase with HIV infection. These data will establish a novel role of exosomes, HIV TAR RNA and immunosuppressive biomarkers in cervical carcinogenesis during HIV infection. This link can be used to identify biomarkers or therapeutic interventions for cervical disease progression during HIV infection.