Cancer is a disease that arises from the shortcomings of the immune system in dealing with faulty cells. In 2020, over 1000 Batswana lives were lost to cancer. Due to the absence of a cure, the most common treatments are chemotherapy and radiotherapy, which may have adverse health side effects. Therefore, there is an urgent, global need to broaden the manners in which cancer is treated. Employing medicinal plants has increased significantly in recent decades with a positive correlation between the intake between plant materials and decrease in several cancers. Documentation about medicinal plants currently being used in Botswana for their anti-cancer properties is limited. However, the indigenous plants are probable sources of anti-cancer agents due to the climate. Once obtained, a need for their rapid analysis is imperative. Currently, the methods of analysis include the phytochemical route, which is costly and involves the use of large quantities of organic solvents. An alternative route could be by chromatography from an analytical chemistry perspective, however the methods available can screen only three compounds simultaneously. This project therefore aims at the development of a novel RP-HPLC method for the simultaneous separation and determination of ten selected anti-cancer compounds found in medicinal plants.