Background: Tenofovir disoproxil fumarate (TDF) is widely used alone or with other antiretroviral drugs for managing Human Immunodeficiency virus (HIV). TDF is often used as prophylaxis because it is highly effective in reducing the risk of sexual acquisition or transmission of HIV. However, it has been reported with a low CNS penetration rate and metabolic disorders. Thus, HIV-associated neurocognitive disorders remain high among HIV-positive patients. Silver nanoparticles-TDF conjugate (TDF-AgNPs) could offer an advantage in reducing these disorders and CNS-penetration rate. Thus, this study evaluated the behavioural and hippocampal ultrastructural changes following interaction with TDF-silver nanoparticles conjugate in diabetic rats.

Methods: Forty-two adult male Sprague-Dawley rats (250 ± 13 g) were randomly divided into non-diabetic groups (1–3) and diabetic groups (4–6), each administered distilled water (0.5 ml/100g, p.o), TDF (26.8 mg/kg/bw, p.o) or TDF-AgNPs (6.7 mg/kg, i.p). After 8 weeks of administration, cognitive function, oxidative injury, and tissue inflammation were evaluated. Furthermore, Hippocampal ultrastructure was observed using transmission electron microscopy, Results: The Nissl staining, and immunohistochemistry. Diabetic rats administered TDF exhibited cognitive deficits; and increases in blood glucose, malondialdehyde, and interleukin-1 beta (IL-1β) levels, which correlate with decreases in glutathione (GSH) and superoxide dismutase (SOD) and catalase (CAT) activities. Furthermore, loss of Hippocampal astrocytic and neuronal organelles were observed. Interestingly, TDF-AgNPs administration to diabetic rats improved cognitive deficits; and increased GSH, SOD, and CAT but reduced Hippocampal malondialdehyde, TNF-α and IL-1β concentrations. Notably, AgNPs-TDF prevented the loss of Hippocampal neuronal and astrocytic cells and the morphology aberration of neuronal organelles.

Conclusion: This study revealed that TDF-AgNPs attenuated cognitive deficits via silver nanoparticles’ antioxidant and anti-inflammatory properties and prevented the loss of Hippocampal astrocytic and neuronal cells.

Background: Tenofovir disoproxil fumarate (TDF) is widely used alone or with other antiretroviral drugs for managing Human Immunodeficiency virus (HIV). TDF is often used as prophylaxis because it is highly effective in reducing the risk of sexual acquisition or transmission of HIV. However, it has been reported with a low CNS penetration rate and metabolic disorders. Thus, HIV-associated neurocognitive disorders remain high among HIV-positive patients. Silver nanoparticles-TDF conjugate (TDF-AgNPs) could offer an advantage in reducing these disorders and CNS-penetration rate. Thus, this study evaluated the behavioural and hippocampal ultrastructural changes following interaction with TDF-silver nanoparticles conjugate in diabetic rats.

Methods: Forty-two adult male Sprague-Dawley rats (250 ± 13 g) were randomly divided into non-diabetic groups (1–3) and diabetic groups (4–6), each administered distilled water (0.5 ml/100g, p.o), TDF (26.8 mg/kg/bw, p.o) or TDF-AgNPs (6.7 mg/kg, i.p). After 8 weeks of administration, cognitive function, oxidative injury, and tissue inflammation were evaluated. Furthermore, Hippocampal ultrastructure was observed using transmission electron microscopy, Results: The Nissl staining, and immunohistochemistry. Diabetic rats administered TDF exhibited cognitive deficits; and increases in blood glucose, malondialdehyde, and interleukin-1 beta (IL-1β) levels, which correlate with decreases in glutathione (GSH) and superoxide dismutase (SOD) and catalase (CAT) activities. Furthermore, loss of Hippocampal astrocytic and neuronal organelles were observed. Interestingly, TDF-AgNPs administration to diabetic rats improved cognitive deficits; and increased GSH, SOD, and CAT but reduced Hippocampal malondialdehyde, TNF-α and IL-1β concentrations. Notably, AgNPs-TDF prevented the loss of Hippocampal neuronal and astrocytic cells and the morphology aberration of neuronal organelles.

Conclusion: This study revealed that TDF-AgNPs attenuated cognitive deficits via silver nanoparticles’ antioxidant and anti-inflammatory properties and prevented the loss of Hippocampal astrocytic and neuronal cells.

Projects

Currently no objects available

Currently no objects available